Characterization and whole genome analysis of human papillomavirus type 16 e1-1374^63nt variants.

ABSTRACT:

Background

The variation of the most common Human papillomavirus (HPV) type found in cervical cancer, the HPV16, has been extensively investigated in almost all viral genes. The E1 gene variation, however, has been rarely studied. The main objective of the present investigation was to analyze the variability of the E6 and E1 genes, focusing on the recently identified E1-1374^63nt variant.

Methodology/principal findings

Variation within the E6 of 786 HPV16 positive cervical samples was analyzed using high-resolution melting, while the E1-1374^63nt duplication was assayed by PCR. Both techniques were supplemented with sequencing. The E1-1374^63nt duplication was linked with the E-G350 and the E-C109/G350 variants. In comparison to the referent HPV16, the E1-1374^63nt E-G350 va

SUBMITTER: Sabol I 

PROVIDER: S-EPMC3404080 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature