Sequence analysis for detection of first-line drug resistance in Mycobacterium tuberculosis strains from a high-incidence setting.

ABSTRACT:

Background

Drug resistance displays a problem for the therapy of Mycobacterium tuberculosis infections. For molecular resistance testing, it is essential to have precise knowledge on genomic variations involved in resistance development. However, data from high-incidence settings are only sparely available. Therefore we performed a systematic approach and analyzed a total of 97 M. tuberculosis strains from previously treated patients in Sierra Leone for mutations in katG, rpoB, rrs, rpsL, gidB, embB, pncA and where applicable in inhA and ahpC. Of the strains investigated 50 were either mono- or poly-resistant to isoniazid, rifampin, streptomycin, ethambutol and pyrazinamide or MDR and 47 fully susceptible strains served as controls.

Results

The majority of isoniazid and rifa

SUBMITTER: Feuerriegel S 

PROVIDER: S-EPMC3404943 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature