Glutamatergic and GABAergic metabolism in mouse brain under chronic nicotine exposure: implications for addiction.

ABSTRACT:

Background and purpose

The effects of nicotine on cerebral metabolism and its influence on smoking behavior is poorly understood. An understanding of the chronic effects of nicotine on excitatory and inhibitory metabolic demand, and corresponding neurotransmission may provide clues for designing strategies for the optimal smoking cessation intervention. The objective of the current study was to investigate neuronal and astroglial metabolism in mice exposed to nicotine (0.5 and 2.0 mg/kg, s.c.) three times in a day for 4 weeks.

Experimental approach/principal findings

Metabolic measurements were carried out by co-infusing [U-(13)C(6)]glucose and [2-(13)C]acetate, and monitoring (13)C labeling of amino acids in brain tissue extract using (1)H-[(13)C] and (13)C-[(1)H]-NMR spect

SUBMITTER: Shameem M 

PROVIDER: S-EPMC3405019 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature