A modular toolset for recombination transgenesis and neurogenetic analysis of Drosophila.

ABSTRACT: Transgenic Drosophila have contributed extensively to our understanding of nervous system development, physiology and behavior in addition to being valuable models of human neurological disease. Here, we have generated a novel series of modular transgenic vectors designed to optimize and accelerate the production and analysis of transgenes in Drosophila. We constructed a novel vector backbone, pBID, that allows both phiC31 targeted transgene integration and incorporates insulator sequences to ensure specific and uniform transgene expression. Upon this framework, we have built a series of constructs that are either backwards compatible with existing restriction enzyme based vectors or utilize Gateway recombination technology for high-throughput cloning. These vectors allow for endogenous pr

SUBMITTER: Wang JW 

PROVIDER: S-EPMC3405054 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature