Dynamic expression of the translational machinery during Bacillus subtilis life cycle at a single cell level.

ABSTRACT: The ability of bacteria to responsively regulate the expression of translation components is crucial for rapid adaptation to fluctuating environments. Utilizing Bacillus subtilis (B. subtilis) as a model organism, we followed the dynamics of the translational machinery at a single cell resolution during growth and differentiation. By comprehensive monitoring the activity of the major rrn promoters and ribosomal protein production, we revealed diverse dynamics between cells grown in rich and poor medium, with the most prominent dissimilarities exhibited during deep stationary phase. Further, the variability pattern of translational activity varied among the cells, being affected by nutrient availability. We have monitored for the first time translational dynamics during the developmental pr

SUBMITTER: Rosenberg A 

PROVIDER: S-EPMC3405057 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature