Parameterization of solvent-protein interaction and its use on NMR protein structure determination.

ABSTRACT: NMR structure determination is frequently hindered by an insufficient amount of distance information for determining the correct fold of the protein in its early stages. In response we introduce a simple and general structure-based metric that can be used to incorporate NMR-based restraints on protein surface accessibility. This metric is inversely proportional to the sum of the inverse square distances to neighboring heavy atoms. We demonstrate the use of this restraint using a dataset from the water to protein magnetization transfer experiment on the protein Bax and the solvent paramagnetic relaxation enhancement experiment on the protein ubiquitin and Qua1 homodimer. The calculated solvent accessibility values using the new empirical function are well correlated with the experimental da

SUBMITTER: Wang Y 

PROVIDER: S-EPMC3405189 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature