Visualisation of J-type counter-current chromatography: a route to understand hydrodynamic phase distribution and retention.

ABSTRACT: This paper has addressed decade sought-after questions on phase bilateral distribution and stationary phase retention in any J-type high-speed counter-current chromatographic (CCC) centrifuge. Using a 2-D spiral column operated on such a CCC device and an aqueous two-phase system, this work systematically observed the phase interaction during transitional period and at dynamic equilibration under stroboscopic illumination. The experimental results thus obtained were used to examine the effects of the liquid-solid friction force, tangential centrifugal force, and physical properties of the two-phase system on hydrodynamic phase behaviour. We identified that (a) density difference between lower and upper phases is the critical factor to cause unusual phase bilateral distribution in the 2-D s

SUBMITTER: Guan YH 

PROVIDER: S-EPMC3405518 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature