Sequetyping: serotyping Streptococcus pneumoniae by a single PCR sequencing strategy.

ABSTRACT: The introduction of pneumococcal conjugate vaccines necessitates continued monitoring of circulating strains to assess vaccine efficacy and replacement serotypes. Conventional serological methods are costly, labor-intensive, and prone to misidentification, while current DNA-based methods have limited serotype coverage requiring multiple PCR primers. In this study, a computer algorithm was developed to interrogate the capsulation locus (cps) of vaccine serotypes to locate primer pairs in conserved regions that border variable regions and could differentiate between serotypes. In silico analysis of cps from 92 serotypes indicated that a primer pair spanning the regulatory gene cpsB could putatively amplify 84 serotypes and differentiate 46. This primer set was specific to Streptococcus pneum

SUBMITTER: Leung MH 

PROVIDER: S-EPMC3405617 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature