Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.

ABSTRACT: Functional studies will facilitate characterization of role and essentiality of newly available genome sequences of the human schistosomes, Schistosoma mansoni, S. japonicum and S. haematobium. To develop transgenesis as a functional approach for these pathogens, we previously demonstrated that pseudotyped murine leukemia virus (MLV) can transduce schistosomes leading to chromosomal integration of reporter transgenes and short hairpin RNA cassettes. Here we investigated vertical transmission of transgenes through the developmental cycle of S. mansoni after introducing transgenes into eggs. Although MLV infection of schistosome eggs from mouse livers was efficient in terms of snail infectivity, >10-fold higher transgene copy numbers were detected in cercariae derived from in vitro laid eggs

SUBMITTER: Rinaldi G 

PROVIDER: S-EPMC3406096 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature