Exploiting a natural auxotrophy for genetic selection.

ABSTRACT: We exploited the natural histidine auxotrophy of Francisella species to develop hisD (encodes histidinol dehydrogenase) as a positive selection marker. A shuttle plasmid (pBR103) carrying Escherichia coli hisD and designed for cloning of PCR fragments replicated in both attenuated and highly virulent Francisella strains. During this work, we formulated a simplified defined growth medium for Francisella novicida.

SUBMITTER: Ramage E 

PROVIDER: S-EPMC3406110 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature