Raman spectroscopy as a potential tool for detection of Brucella spp. in milk.

ABSTRACT: Detection of Brucella, causing brucellosis, is very challenging, since the applied techniques are mostly time-demanding and not standardized. While the common detection system relies on the cultivation of the bacteria, further classical typing up to the biotype level is mostly based on phenotypic or genotypic characteristics. The results of genotyping do not always fit the existing taxonomy, and misidentifications between genetically closely related genera cannot be avoided. This situation gets even worse, when detection from complex matrices, such as milk, is necessary. For these reasons, the availability of a method that allows early and reliable identification of possible Brucella isolates for both clinical and epidemiological reasons would be extremely useful. We evaluated micro-Raman

SUBMITTER: Meisel S 

PROVIDER: S-EPMC3406119 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature