Analysis of hepcidin expression: in situ hybridization and quantitative polymerase chain reaction from paraffin sections.

ABSTRACT:

Aim

To establish methods for quantitative polymerase chain reaction (PCR) for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of hepatocellular carcinoma (HCC).

Methods

Total RNA from paraffin-embedded sections was isolated from 68 paraffin-embedded samples of HCC. Samples came from 54 male and 14 female patients with a mean age of 66.8 ± 7.8 years. Quantitative PCR was performed. Immunohistochemistry and in situ hybridization for hepcidin were also performed.

Results

Quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections of HCC was performed successfully. The expression level of hepcidin mRNA in cancer tissues was significantly higher than that in non-cancer tissues. A method of in situ hybridization f

SUBMITTER: Sakuraoka Y 

PROVIDER: S-EPMC3406426 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature