Screening the pathogenic genes and pathways related to DMBA (7,12-dimethylbenz[a]anthracene)-induced transformation of hamster oral mucosa from precancerous lesions to squamous cell carcinoma.

ABSTRACT: This study aimed to screen the pathogenic genes and pathways that relate to the transformation of hamster buccal mucosa from precancerous lesions to squamous cell carcinoma by whole genome microarray and bioinformatics analysis. A DMBA (7,12-dimethylbenz[a]anthracene)-induced hamster model of a precancerous lesion and squamous cell carcinoma was established. The differentially expressed genes were detected using an Agilent whole rat genome microarray, which contains 41,000 genes/ESTs. Gene ontology (GO) functional classification and pathway analyses were performed, and a subset of differentially expressed genes were validated using RT-PCR. The results showed that during the transformation of hamster buccal mucosa from the precancerous lesion to squamous cell carcinoma, a total of 1,981 gen

SUBMITTER: Chen D 

PROVIDER: S-EPMC3406442 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature