Human fetal skeletal muscle contains a myogenic side population that expresses the melanoma cell-adhesion molecule.

ABSTRACT: Muscle side population (SP) cells are rare myogenic progenitors distinct from satellite cells, the known tissue-specific stem cells of skeletal muscle. Studies in mice demonstrated that muscle SP cells give rise to satellite cells in vivo. Given that muscle SP cells are heterogeneous, it has been difficult to prospectively enrich for myogenic progenitors within the SP fraction, particularly from human tissue. Further, conditions that favor the expansion of human muscle SP cells while retaining their myogenic potential have yet to be reported. In this study, human fetal muscle SP and main population (MP) cells were purified based on the expression of melanoma cell adhesion molecule (MCAM), a marker we previously reported to enrich for cells with myogenic potential. To define the relationshi

SUBMITTER: Lapan AD 

PROVIDER: S-EPMC3406760 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature