The importance of 1H-nuclear magnetic resonance spectroscopy for reference standard validation in analytical sciences.

ABSTRACT: This paper highlights the importance of recording at least a (1)H nuclear magnetic resonance (NMR) spectrum to verify identity of standards used in analyses of organic materials irrespective of source. We show the importance of this approach with an example of a quantitative high-performance liquid chromatography (HPLC) study undertaken with green tea extracts that required the use of several polyphenols as standards. In the course of the study one of these standards [(-)-epigallocatechin, EGC], although having the physical appearance and appropriate HPLC chromatographic behavior of EGC, proved by (1)H-NMR to be a completely different class of molecule. For us, this raised significant questions concerning validity of many published pieces of research that used quantitative HPLC methods wit

SUBMITTER: Kelman D 

PROVIDER: S-EPMC3407044 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature