Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.

ABSTRACT:

Background

Intestinal ischemia-reperfusion (I/R) plays an important role in critical illnesses. Gut flora participate in the pathogenesis of the injury. This study is aimed at unraveling colonic microbiota alteration pattern and identifying specific bacterial species that differ significantly as well as observing colonic epithelium change in the same injury model during the reperfusion time course.

Methodology/principal findings

Denaturing gradient gel electrophoresis (DGGE) was used to monitor the colonic microbiota of control rats and experimental rats that underwent 0.5 hour ischemia and 1, 3, 6, 12, 24, and 72 hours following reperfusion respectively. The microbiota similarity, bacterial diversity and species that characterized the dysbiosis were estimated based on the D

SUBMITTER: Wang F 

PROVIDER: S-EPMC3407053 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature