Discovery of novel dsRNA viral sequences by in silico cloning and implications for viral diversity, host range and evolution.

ABSTRACT: Genome sequence of viruses can contribute greatly to the study of viral evolution, diversity and the interaction between viruses and hosts. Traditional molecular cloning methods for obtaining RNA viral genomes are time-consuming and often difficult because many viruses occur in extremely low titers. DsRNA viruses in the families, Partitiviridae, Totiviridae, Endornaviridae, Chrysoviridae, and other related unclassified dsRNA viruses are generally associated with symptomless or persistent infections of their hosts. These characteristics indicate that samples or materials derived from eukaryotic organisms used to construct cDNA libraries and EST sequencing might carry these viruses, which were not easily detected by the researchers. Therefore, the EST databases may include numerous unknown v

SUBMITTER: Liu H 

PROVIDER: S-EPMC3407116 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature