System level changes in gene expression in maturing bladder mucosa.

ABSTRACT:

Purpose

Bladder problems clinically present early in life as birth defects that often lead to kidney failure and late in life as overactive bladder, incontinence and related disorders. We investigated the transcriptome of mouse bladder mucosa at juvenile and adult stages by microarray to identify the pathways associated with normal, healthy growth and maturation. We hypothesized that understanding these pathways could be key to achieving bladder regeneration or reawakening normal function in the elderly population.

Materials and methods

RNA was isolated from the mucosa at 3, 6, 20 and 30 weeks postnatally. Affymetrix® Mouse 430 v2 arrays were used to profile the expression of approximately 45,000 genes. The software program Statistical Analysis of Microarrays was used to ide

SUBMITTER: Dozmorov M 

PROVIDER: S-EPMC3407683 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature