A genomic approach to investigate expression profiles in Slovenian patients with gastric cancer.

ABSTRACT: Despite its decreasing frequency in developed countries, gastric cancer remains a significant health burden. The aim of the present study was to construct cDNA libraries and analyze differentially expressed genes related to this disease. Gene expression profiles were generated with suppressive subtraction hybridization (SSH). We constructed eight SSH libraries, four representing up-regulated genes and four representing down-regulated genes in tumor tissues. Our approach revealed that several genes are abnormally expressed in gastric cancer. We also identified global deregulation of several pathways involved in the maintenance of normal gastric homeostasis. The results of this study support the view that, as a result of complex pathogenesis, diversity of genomic aberrations and multiplicity

SUBMITTER: Hudler P 

PROVIDER: S-EPMC3408025 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature