Coculture with hematopoietic stem cells protects cardiomyocytes against apoptosis via paracrine activation of AKT.

ABSTRACT:

Background

Previous experimental studies concluded that stem cells (SC) may exert their beneficial effects on the ischemic heart by paracrine activation of antiapoptotic pathways. In order to identify potential cardioprotective mediators, we performed a systematic analysis of the differential gene expression of hematopoietic SC after coculture with cardiomyocytes (CM).

Methods

After 48 h of coculture with neonatal rat ventricular CM (NRVCM), two consecutive cell sorting steps generated a highly purified population of conditioned murine hematopoietic SC (>99%). Next, a genome-wide microarray analysis of cocultured vs. monocultured hematopoietic SC derived from three independent experiments was performed. The analysis of differentially expressed genes was focused on products t

SUBMITTER: Rosenberg M 

PROVIDER: S-EPMC3408384 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature