Comparative transcriptional analysis of clinically relevant heat stress response in Clostridium difficile strain 630.

ABSTRACT: Clostridium difficile is considered to be one of the most important causes of health care-associated infections worldwide. In order to understand more fully the adaptive response of the organism to stressful conditions, we examined transcriptional changes resulting from a clinically relevant heat stress (41 °C versus 37 °C) in C. difficile strain 630 and identified 341 differentially expressed genes encompassing multiple cellular functional categories. While the transcriptome was relatively resilient to the applied heat stress, we noted upregulation of classical heat shock genes including the groEL and dnaK operons in addition to other stress-responsive genes. Interestingly, the flagellin gene (fliC) was downregulated, yet genes encoding the cell-wall associated flagellar components were u

SUBMITTER: Ternan NG 

PROVIDER: S-EPMC3408451 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature