Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps.

ABSTRACT:

Background

Although chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) is characterized by T(H)2 inflammation, the role of mast cells is poorly understood.

Objective

The objective of this study was to investigate the presence, localization, and phenotype of mast cells in patients with CRS.

Methods

We collected nasal tissue and nasal lavage fluid from patients with CRS and control subjects. We analyzed mRNA for the mast cell proteases tryptase, chymase, and carboxypeptidase A3 by using real-time PCR and measured mast cell protease proteins by using ELISA, immunohistochemistry, and immunofluorescence.

Results

Tryptase mRNA was significantly increased in nasal polyps (NPs) from patients with CRSwNP (P< .001) compared with uncinate tissue from patients with CR

SUBMITTER: Takabayashi T 

PROVIDER: S-EPMC3408832 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature