Identification and characterization of argonaute protein, Ago2 and its associated small RNAs in Schistosoma japonicum.

ABSTRACT:

Background

The complex life cycle of the genus Schistosoma drives the parasites to employ subtle developmentally dependent gene regulatory machineries. Small non-coding RNAs (sncRNAs) are essential gene regulatory factors that, through their impact on mRNA and genome stability, control stage-specific gene expression. Abundant sncRNAs have been identified in this genus. However, their functionally associated partners, Argonaute family proteins, which are the key components of the RNA-induced silencing complex (RISC), have not yet been fully explored.

Methodology/principal findings

Two monoclonal antibodies (mAbs) specific to Schistosoma japonicum Argonaute protein Ago2 (SjAgo2), but not SjAgo1 and SjAgo3, were generated. Soluble adult worm antigen preparation (SWAP) was subje

SUBMITTER: Cai P 

PROVIDER: S-EPMC3409120 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature