Deep Panning: steps towards probing the IgOme.

ABSTRACT:

Background

Polyclonal serum consists of vast collections of antibodies, products of differentiated B-cells. The spectrum of antibody specificities is dynamic and varies with age, physiology, and exposure to pathological insults. The complete repertoire of antibody specificities in blood, the IgOme, is therefore an extraordinarily rich source of information-a molecular record of previous encounters as well as a status report of current immune activity. The ability to profile antibody specificities of polyclonal serum at exceptionally high resolution has been an important and serious challenge which can now be overcome.

Methodology/principal findings

Here we illustrate the application of Deep Panning, a method that combines the flexibility of combinatorial phage display of ran

SUBMITTER: Ryvkin A 

PROVIDER: S-EPMC3409857 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature