Identification of soybean seed developmental stage-specific and tissue-specific miRNA targets by degradome sequencing.

ABSTRACT:

Background

MicroRNAs (miRNAs) regulate the expression of target genes by mediating gene silencing in both plants and animals. The miRNA targets have been extensively investigated in Arabidopsis and rice using computational prediction, experimental validation by overexpression in transgenic plants, and by degradome or PARE (parallel analysis of RNA ends) sequencing. However, miRNA targets mostly remain unknown in soybean (Glycine max). More specifically miRNA mediated gene regulation at different seed developmental stages in soybean is largely unexplored. In order to dissect miRNA guided gene regulation in soybean developing seeds, we performed a transcriptome-wide experimental method using degradome sequencing to directly detect cleaved miRNA targets.

Results

In this study,

SUBMITTER: Shamimuzzaman M 

PROVIDER: S-EPMC3410764 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature