HIV-1-encoded antisense RNA suppresses viral replication for a prolonged period.

ABSTRACT:

Background

Recent evidence proposes a novel concept that mammalian natural antisense RNAs play important roles in cellular homeostasis by regulating the expression of several genes. Identification and characterization of retroviral antisense RNA would provide new insights into mechanisms of replication and pathogenesis. HIV-1 encoded-antisense RNAs have been reported, although their structures and functions remain to be studied. We have tried to identify and characterize antisense RNAs of HIV-1 and their function in viral infection.

Results

Characterization of transcripts of HEK293T cells that were transiently transfected with an expression plasmid with HIV-1NL4-3 DNA in the antisense orientation showed that various antisense transcripts can be expressed. By screening and ch

SUBMITTER: Kobayashi-Ishihara M 

PROVIDER: S-EPMC3410806 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature