Calcium influx measured at single presynaptic boutons of cerebellar granule cell ascending axons and parallel fibers.

ABSTRACT: Action potential-evoked calcium influx into presynaptic boutons is a key determinant of synaptic strength and function. Here, we have examined the calcium dynamics at individual presynaptic boutons of the cerebellar granule cells in the molecular layer of cerebellar slices and investigated whether different subpopulations of granule cell boutons exhibit different calcium dynamics. We found that a population of boutons with low basal calcium clearance rates may activate a second clearance mechanism and exhibit biphasic calcium decay on high calcium influx induced by bursts of action potentials. We also found that boutons on ascending axons and parallel fibers show similar calcium influx amplitudes and calcium clearance rates in response to action potentials. Lastly, we found that parallel f

SUBMITTER: Zhang W 

PROVIDER: S-EPMC3411191 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature