Predominant role of active versus facilitative glucose transport for glucagon-like peptide-1 secretion.

ABSTRACT:

Aims/hypothesis

Several glucose-sensing pathways have been implicated in glucose-triggered secretion of glucagon-like peptide-1 (GLP-1) from intestinal L cells. One involves glucose metabolism and closure of ATP-sensitive K(+) channels, and another exploits the electrogenic nature of Na(+)-coupled glucose transporters (SGLTs). This study aimed to elucidate the role of these distinct mechanisms in glucose-stimulated GLP-1 secretion.

Methods

Glucose uptake into L cells (either GLUTag cells or cells in primary cultures, using a new transgenic mouse model combining proglucagon promoter-driven Cre recombinase with a ROSA26tdRFP reporter) was monitored with the FLII(12)Pglu-700 μδ6 glucose sensor. Effects of pharmacological and genetic interference with SGLT1 or facilitative gluco

SUBMITTER: Parker HE 

PROVIDER: S-EPMC3411305 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature