Transcriptome analysis of carnation (Dianthus caryophyllus L.) based on next-generation sequencing technology.

ABSTRACT:

Background

Carnation (Dianthus caryophyllus L.), in the family Caryophyllaceae, can be found in a wide range of colors and is a model system for studies of flower senescence. In addition, it is one of the most important flowers in the global floriculture industry. However, few genomics resources, such as sequences and markers are available for carnation or other members of the Caryophyllaceae. To increase our understanding of the genetic control of important characters in carnation, we generated an expressed sequence tag (EST) database for a carnation cultivar important in horticulture by high-throughput sequencing using 454 pyrosequencing technology.

Results

We constructed a normalized cDNA library and a 3'-UTR library of carnation, obtaining a total of 1,162,126 high-quali

SUBMITTER: Tanase K 

PROVIDER: S-EPMC3411436 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature