Hypoxia-induced alternative splicing in endothelial cells.

ABSTRACT:

Background

Adaptation to low oxygen by changing gene expression is vitally important for cell survival and tissue development. The sprouting of new blood vessels, initiated from endothelial cells, restores the oxygen supply of ischemic tissues. In contrast to the transcriptional response induced by hypoxia, which is mainly mediated by members of the HIF family, there are only few studies investigating alternative splicing events. Therefore, we performed an exon array for the genome-wide analysis of hypoxia-related changes of alternative splicing in endothelial cells.

Methodology/principal findings

Human umbilical vein endothelial cells (HUVECs) were incubated under hypoxic conditions (1% O(2)) for 48 h. Genome-wide transcript and exon expression levels were assessed using th

SUBMITTER: Weigand JE 

PROVIDER: S-EPMC3411717 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature