Characterization of Na+ and Ca2+ channels in zebrafish dorsal root ganglion neurons.

ABSTRACT:

Background

Dorsal root ganglia (DRG) somata from rodents have provided an excellent model system to study ion channel properties and modulation using electrophysiological investigation. As in other vertebrates, zebrafish (Danio rerio) DRG are organized segmentally and possess peripheral axons that bifurcate into each body segment. However, the electrical properties of zebrafish DRG sensory neurons, as compared with their mammalian counterparts, are relatively unexplored because a preparation suitable for electrophysiological studies has not been available.

Methodology/principal findings

We show enzymatically dissociated DRG neurons from juvenile zebrafish expressing Isl2b-promoter driven EGFP were easily identified with fluorescence microscopy and amenable to conventional wh

SUBMITTER: Won YJ 

PROVIDER: S-EPMC3411820 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature