Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells.

ABSTRACT: Hepatocytes generated from human induced pluripotent stem cells (hiPSCs) are unprecedented resources for pharmaceuticals and cell therapy. However, the in vitro directed differentiation of human pluripotent stem cells into mature hepatocytes remains challenging. Little attention has so far been paid to variations among hiPSC lines in terms of their hepatic differentiation. In the current study, we developed an improved hepatic differentiation protocol and compared 28 hiPSC lines originated from various somatic cells and derived using retroviruses, Sendai viruses, or episomal plasmids. This comparison indicated that the origins, but not the derivation methods, may be a major determinant of variation in hepatic differentiation. The hiPSC clones derived from peripheral blood cells consistentl

SUBMITTER: Kajiwara M 

PROVIDER: S-EPMC3411998 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature