Distance dependent interhelical couplings of organic rods incorporated in DNA 4-helix bundles.

ABSTRACT: The synthesis of a conjugated linear organic module containing terminal salicylaldehyde groups and a central activated ester, designed for conjugation to amino-modified oligonucleotides, is presented. The organic module has a phenylene-ethynylene backbone and is highly fluorescent. It is conjugated to oligonucleotide sequences and incorporated into specific locations in a well-defined DNA 4-helix bundle (4-HB). The DNA-nanostructure offers precise location control of the organic modules which allows for selective interhelical coupling reactions. In this study, metal-salen formation as well as dihydrazone formation are used to covalently interlink the organic modules. Both coupling reactions are highly dependent on the distances between the organic modules in the 4-HB. Neighboring modules d

SUBMITTER: Andersen CS 

PROVIDER: S-EPMC3412156 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature