A distinct expression pattern of the long 3'-untranslated region dicer mRNA and its implications for posttranscriptional regulation in colorectal cancer.

ABSTRACT:

Objectives

Reduced expression of Dicer is associated with global downregulation of microRNAs. Primary Dicer transcripts can be processed at two alternative polyadenylation sites, generating two pools of messenger RNAs (mRNAs) that carry either a long or a short 3'-untranslated regions (3'UTRs), that both encode the same Dicer protein. The short 3'UTR Dicer mRNA is not regulated by miR-103/107. The aim of this study was to investigate the expression of total Dicer mRNA, long 3'UTR Dicer mRNA, and miR-103 in colorectal cancer (CRC).

Methods

Paired tumor and normal mucosal specimens were obtained from 66 patients with CRC. Real-time reverse transcription PCR of long 3'UTR Dicer mRNA, total Dicer mRNA and miR-103 was carried out using the TaqMan Expression assay and the TaqMan M

SUBMITTER: Hamaya Y 

PROVIDER: S-EPMC3412679 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature