Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.

ABSTRACT:

Background

Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and within certain distances that allow undesired amplification. The process of designing specific primers typically involves two stages. First, the primers flanking regions of interest are generated either manually or using software tools; then they are searched against an appropriate nucleotide sequence database using tools such as BLAST to examine the potential targets. However, the latter is not an easy process as one needs to examine many details between primers and targets, such as the number and the positions of matche

SUBMITTER: Ye J 

PROVIDER: S-EPMC3412702 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature