Alu expression in human cell lines and their retrotranspositional potential.

ABSTRACT:

Background

The vast majority of the 1.1 million Alu elements are retrotranspositionally inactive, where only a few loci referred to as 'source elements' can generate new Alu insertions. The first step in identifying the active Alu sources is to determine the loci transcribed by RNA polymerase III (pol III). Previous genome-wide analyses from normal and transformed cell lines identified multiple Alu loci occupied by pol III factors, making them candidate source elements.

Findings

Analysis of the data from these genome-wide studies determined that the majority of pol III-bound Alus belonged to the older subfamilies Alu S and Alu J, which varied between cell lines from 62.5% to 98.7% of the identified loci. The pol III-bound Alus were further scored for estimated retrotransposi

SUBMITTER: Oler AJ 

PROVIDER: S-EPMC3412727 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature