In vitro generation of monocyte-derived macrophages under serum-free conditions improves their tumor promoting functions.

ABSTRACT: The tumor promoting role of M2 macrophages has been described in in vivo models and the presence of macrophages in certain tumor types has been linked to a poor clinical outcome. In light of burgeoning activities to clinically develop new therapies targeting tumor-associated macrophages (TAMs), reliable in vitro models faithfully mimicking the tumor promoting functions of TAMs are required. Generation and activation of human monocyte-derived macrophages (MDM) in vitro, described as M1 or M2 macrophages attributed with tumoricidal or tumor-promoting functions, respectively, has been widely reported using mainly serum containing culture methods. In this study, we compared the properties of macrophages originating from monocytes cultured either in media containing serum together with M-CSF fo

SUBMITTER: Rey-Giraud F 

PROVIDER: S-EPMC3412794 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature