Transcriptome analysis and SSR/SNP markers information of the blunt snout bream (Megalobrama amblycephala).

ABSTRACT:

Background

Blunt snout bream (Megalobrama amblycephala) is an herbivorous freshwater fish species native to China and has been recognized as a main aquaculture species in the Chinese freshwater polyculture system with high economic value. Right now, only limited EST resources were available for M. amblycephala. Recent advances in large-scale RNA sequencing provide a fast, cost-effective, and reliable approach to generate large expression datasets for functional genomic analysis, which is especially suitable for non-model species with un-sequenced genomes.

Methodology and principal findings

Using 454 pyrosequencing, a total of 1,409,706 high quality reads (total length 577 Mbp) were generated from the normalized cDNA of pooled M. amblycephala individuals. These sequences were

SUBMITTER: Gao Z 

PROVIDER: S-EPMC3412804 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature