DNA origami as biocompatible surface to match single-molecule and ensemble experiments.

ABSTRACT: Single-molecule experiments on immobilized molecules allow unique insights into the dynamics of molecular machines and enzymes as well as their interactions. The immobilization, however, can invoke perturbation to the activity of biomolecules causing incongruities between single molecule and ensemble measurements. Here we introduce the recently developed DNA origami as a platform to transfer ensemble assays to the immobilized single molecule level without changing the nano-environment of the biomolecules. The idea is a stepwise transfer of common functional assays first to the surface of a DNA origami, which can be checked at the ensemble level, and then to the microscope glass slide for single-molecule inquiry using the DNA origami as a transfer platform. We studied the structural flexibi

SUBMITTER: Gietl A 

PROVIDER: S-EPMC3413134 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature