Regulation of DNA replication timing on human chromosome by a cell-type specific DNA binding protein SATB1.

ABSTRACT:

Background

Replication timing of metazoan DNA during S-phase may be determined by many factors including chromosome structures, nuclear positioning, patterns of histone modifications, and transcriptional activity. It may be determined by Mb-domain structures, termed as "replication domains", and recent findings indicate that replication timing is under developmental and cell type-specific regulation.

Methodology/principal findings

We examined replication timing on the human 5q23/31 3.5-Mb segment in T cells and non-T cells. We used two independent methods to determine replication timing. One is quantification of nascent replicating DNA in cell cycle-fractionated stage-specific S phase populations. The other is FISH analyses of replication foci. Although the locations of earl

SUBMITTER: Oda M 

PROVIDER: S-EPMC3413666 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature