Functional coating of liposomes using a folate- polymer conjugate to target folate receptors.

ABSTRACT: Folate-polymer-coated liposomes were developed for targeted chemotherapy using doxorubicin (DXR) as a model drug. Folate-poly(L-lysine) (F-PLL) conjugates with a folate modification degree of 16.7 mol% on epsilon amino groups of PLL were synthesized. DXR-loaded anionic liposomes were coated with F-PLL, and the cellular association of F-PLL-coated liposomes was evaluated by flow cytometry, and confocal microscopy in human nasopharyngeal carcinoma KB cells overexpressing folate receptors (FRs), and human lung adenocarcinoma A549 cells [FR (-)]. The existence of a polymer layer on the surface of F-PLL-coated liposomes was confirmed by zeta potential analysis. The KB cellular association of F-PLL-coated liposomal DXR was increased compared with that of PLL-coated liposomes and was inhibited in

SUBMITTER: Watanabe K 

PROVIDER: S-EPMC3414201 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature