Comparative analysis of single-species and polybacterial wound biofilms using a quantitative, in vivo, rabbit ear model.

ABSTRACT:

Introduction

The recent literature suggests that chronic wound biofilms often consist of multiple bacterial species. However, without appropriate in vivo, polybacterial biofilm models, our understanding of these complex infections remains limited. We evaluate and compare the effect of single- and mixed-species biofilm infections on host wound healing dynamics using a quantitative, in vivo, rabbit ear model.

Methods

Six-mm dermal punch wounds in New Zealand rabbit ears were inoculated with Staphylococcus aureus strain UAMS-1, Pseudomonas aeruginosa strain PAO1, or both, totaling 10/6 colony-forming units/wound. Bacterial proliferation and maintenance in vivo were done using procedures from our previously published model. Wounds were harvested for histological measurement of w

SUBMITTER: Seth AK 

PROVIDER: S-EPMC3414496 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature