Towards a more accurate annotation of tyrosine-based site-specific recombinases in bacterial genomes.

ABSTRACT:

Background

Tyrosine-based site-specific recombinases (TBSSRs) are DNA breaking-rejoining enzymes. In bacterial genomes, they play a major role in the comings and goings of mobile genetic elements (MGEs), such as temperate phage genomes, integrated conjugative elements (ICEs) or integron cassettes. TBSSRs are also involved in the segregation of plasmids and chromosomes, the resolution of plasmid dimers and of co-integrates resulting from the replicative transposition of transposons. With the aim of improving the annotation of TBSSR genes in genomic sequences and databases, which so far is far from robust, we built a set of over 1,300 TBSSR protein sequences tagged with their genome of origin. We organized them in families to investigate: i) whether TBSSRs tend to be more conserved w

SUBMITTER: Van Houdt R 

PROVIDER: S-EPMC3414803 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature