Pharmacological characterization of rat amylin receptors: implications for the identification of amylin receptor subtypes.

ABSTRACT:

Background and purpose

Amylin (Amy) is an important glucoregulatory peptide and AMY receptors are clinical targets for diabetes and obesity. Human (h) AMY receptor subtypes are complexes of the calcitonin (CT) receptor with receptor activity-modifying proteins (RAMPs); their rodent counterparts have not been characterized. To allow identification of the most clinically relevant receptor subtype, the elucidation of rat (r) AMY receptor pharmacology is necessary.

Experimental approach

Receptors were transiently transfected into COS-7 cells and cAMP responses measured in response to different agonists, with or without antagonists. Competition binding experiments were performed to determine rAmy affinity.

Key results

rCT was the most potent agonist of rCT((a)) receptors,

SUBMITTER: Bailey RJ 

PROVIDER: S-EPMC3415645 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature