Unknown

Dataset Information

0

SNP-Ratio Mapping (SRM): identifying lethal alleles and mutations in complex genetic backgrounds by next-generation sequencing.

ABSTRACT: We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds.

SUBMITTER: Lindner H 

PROVIDER: S-EPMC3416015 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

SNP-Ratio Mapping (SRM): identifying lethal alleles and mutations in complex genetic backgrounds by next-generation sequencing.

Lindner Heike H   Raissig Michael T MT   Sailer Christian C   Shimosato-Asano Hiroko H   Bruggmann Rémy R   Grossniklaus Ueli U  

Genetics 20120529 4

We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds. ...[more]

Similar Datasets

Unknown Improving mapping and SNP-calling performance in multiplexed targeted next-generation sequencing.
| S-EPMC3563481 | biostudies-literature
Unknown ngs_backbone: a pipeline for read cleaning, mapping and SNP calling using next generation sequence.
| S-EPMC3124440 | biostudies-literature
Unknown Discovering lethal alleles across the turkey genome using a transmission ratio distortion approach.
| S-EPMC7702127 | biostudies-literature
Unknown Complete RHD next-generation sequencing: establishment of reference RHD alleles.
| S-EPMC6199663 | biostudies-literature
Unknown Mapping translocation breakpoints by next-generation sequencing.
| S-EPMC2493403 | biostudies-literature
Unknown Genome-wide SNP calling using next generation sequencing data in tomato.
| S-EPMC3907006 | biostudies-literature
Unknown Efficiently identifying genome-wide changes with next-generation sequencing data.
| S-EPMC3201882 | biostudies-literature
Transcriptomics Mapping translocation breakpoints by next-generation sequencing
2010-05-22 | E-GEOD-10115 | biostudies-arrayexpress
Unknown A Bayesian Model for SNP Discovery Based on Next-Generation Sequencing Data.
| S-EPMC4697941 | biostudies-literature
Unknown SNP discovery using Next Generation Transcriptomic Sequencing in Atlantic herring (Clupea harengus).
| S-EPMC3413699 | biostudies-literature