Evaluation of recombinant proteins of Burkholderia mallei for serodiagnosis of glanders.

ABSTRACT: Glanders is a contagious disease caused by the Gram-negative bacillus Burkholderia mallei. The number of equine glanders outbreaks has increased steadily during the last decade. The disease must be reported to the Office International des Epizooties, Paris, France. Glanders serodiagnosis is hampered by the considerable number of false positives and negatives of the internationally prescribed tests. The major problem leading to the low sensitivity and specificity of the complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA) has been linked to the test antigens currently used, i.e., crude preparations of whole cells. False-positive results obtained from other diagnostic tests utilizing crude antigens lead to financial losses to animal owners, and false-negative results

SUBMITTER: Pal V 

PROVIDER: S-EPMC3416092 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature