One-step sequence- and ligation-independent cloning as a rapid and versatile cloning method for functional genomics studies.

ABSTRACT: We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.

SUBMITTER: Jeong JY 

PROVIDER: S-EPMC3416421 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature