A conserved two-component signal transduction system controls the response to phosphate starvation in Bifidobacterium breve UCC2003.

ABSTRACT: This work reports on the identification and molecular characterization of the two-component regulatory system (2CRS) PhoRP, which controls the response to inorganic phosphate (P(i)) starvation in Bifidobacterium breve UCC2003. The response regulator PhoP was shown to bind to the promoter region of pstSCAB, specifying a predicted P(i) transporter system, as well as that of phoU, which encodes a putative P(i)-responsive regulatory protein. This interaction is assumed to cause transcriptional modulation under conditions of P(i) limitation. Our data suggest that the phoRP genes are subject to positive autoregulation and, together with pstSCAB and presumably phoU, represent the complete regulon controlled by the phoRP-encoded 2CRS in B. breve UCC2003. Determination of the minimal PhoP binding r

SUBMITTER: Alvarez-Martin P 

PROVIDER: S-EPMC3416448 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature