Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse model.

ABSTRACT:

Background

Japanese encephalitis virus (JEV) is a major mosquito-borne pathogen that causes viral encephalitis throughout Asia. Vaccination with an inactive JEV particle or attenuated virus is an efficient preventative measure for controlling infection. Flavivirus NS1 protein is a glycoprotein secreted during viral replication that plays multiple roles in the viral life cycle and pathogenesis. Utilizing JEV NS1 as an antigen in viral vectors induces a limited protective immune response against infection. Previous studies using E. coli-expressed JEV NS1 to immunize mice induced protection against lethal challenge; however, the protection mechanism through cellular and humoral immune responses was not described.

Results

JEV NS1 was expressed in and purified from Drosophila S2

SUBMITTER: Li Y 

PROVIDER: S-EPMC3416663 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature