Untranslated regions of diverse plant viral RNAs vary greatly in translation enhancement efficiency.

ABSTRACT:

Background

Whole plants or plant cell cultures can serve as low cost bioreactors to produce massive amounts of a specific protein for pharmacological or industrial use. To maximize protein expression, translation of mRNA must be optimized. Many plant viral RNAs harbor extremely efficient translation enhancers. However, few of these different translation elements have been compared side-by-side. Thus, it is unclear which are the most efficient translation enhancers. Here, we compare the effects of untranslated regions (UTRs) containing translation elements from six plant viruses on translation in wheat germ extract and in monocotyledenous and dicotyledenous plant cells.

Results

The highest expressing uncapped mRNAs contained viral UTRs harboring Barley yellow dwarf virus (BYD

SUBMITTER: Fan Q 

PROVIDER: S-EPMC3416697 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature